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rnascope probe m. musculus rspo1  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope probe m. musculus rspo1
    ( A ) Native EGFP protein expression in frontal cryosection of a P14 Sox2 Egfp/+ pituitary. Schematic of the workflow used for bulk RNA-sequencing analysis of Sox2 + and Sox2 − cells. Genome browser views of reads aligning to the Sox2 and Pit1 loci in the positive and negative fractions indicating good separation of the EGFP + population. Scale bar: 50 µm. ( B ) Sox2 + cells express a significant enrichment in markers associated with epithelial-to-mesenchymal transition (EMT), adherens, and tight junctions, consistent with their epithelial nature. Gene set enrichment analysis (GSEA) plots and immunofluorescence staining against E-Cadherin (adherens junction marker) and ZO1 (tight junction marker) in the marginal zone epithelium at P14. Scale bar: 50 µm. See for full GSEA gene lists. ( C ) Sox2 + cells express a significant enrichment in several signalling pathways, shown with respective GSEA plots. See for full GSEA gene lists. ( D ) Bar charts showing the FPKM values of components of the LGR/RNF43/ZNRF3/R-spondin module in the Sox2 + and Sox2 − fractions and the distribution of the Frizzled receptors. GSEA plot for components of the WNT pathway. Validation of sequencing: (i) mRNA in situ hybridisation with specific probes against Lgr4 (blue) and Sox2 (red) in P14 pituitaries showing co-expression. (ii) Double mRNA in situ hybridisation against Fzd4 (blue) and Sox2 (red) indicating co-expression in both the marginal zone epithelium and parenchymal Sox2 + cells. Boxed regions are magnified. Scale bars: 250 µm and 50 μm in boxed inserts. (iii) mRNA in situ hybridisation against <t>Rspo1</t> , Rspo2 , Rspo3 , and Rspo4 in sagittal sections of wild-type pituitaries at P14. Boxed regions are magnified, only Rspo4 is detected. Scale bars: 250 µm and 100 μm in boxed inserts.
    Rnascope Probe M. Musculus Rspo1, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+probe+m%2E+musculus+rspo1/mouse+rspo1+probe/pmc07803373-41-3-9
    Average 90 stars, based on 1 article reviews
    rnascope probe m. musculus rspo1 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells"

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

    Journal: eLife

    doi: 10.7554/eLife.59142

    ( A ) Native EGFP protein expression in frontal cryosection of a P14 Sox2 Egfp/+ pituitary. Schematic of the workflow used for bulk RNA-sequencing analysis of Sox2 + and Sox2 − cells. Genome browser views of reads aligning to the Sox2 and Pit1 loci in the positive and negative fractions indicating good separation of the EGFP + population. Scale bar: 50 µm. ( B ) Sox2 + cells express a significant enrichment in markers associated with epithelial-to-mesenchymal transition (EMT), adherens, and tight junctions, consistent with their epithelial nature. Gene set enrichment analysis (GSEA) plots and immunofluorescence staining against E-Cadherin (adherens junction marker) and ZO1 (tight junction marker) in the marginal zone epithelium at P14. Scale bar: 50 µm. See for full GSEA gene lists. ( C ) Sox2 + cells express a significant enrichment in several signalling pathways, shown with respective GSEA plots. See for full GSEA gene lists. ( D ) Bar charts showing the FPKM values of components of the LGR/RNF43/ZNRF3/R-spondin module in the Sox2 + and Sox2 − fractions and the distribution of the Frizzled receptors. GSEA plot for components of the WNT pathway. Validation of sequencing: (i) mRNA in situ hybridisation with specific probes against Lgr4 (blue) and Sox2 (red) in P14 pituitaries showing co-expression. (ii) Double mRNA in situ hybridisation against Fzd4 (blue) and Sox2 (red) indicating co-expression in both the marginal zone epithelium and parenchymal Sox2 + cells. Boxed regions are magnified. Scale bars: 250 µm and 50 μm in boxed inserts. (iii) mRNA in situ hybridisation against Rspo1 , Rspo2 , Rspo3 , and Rspo4 in sagittal sections of wild-type pituitaries at P14. Boxed regions are magnified, only Rspo4 is detected. Scale bars: 250 µm and 100 μm in boxed inserts.
    Figure Legend Snippet: ( A ) Native EGFP protein expression in frontal cryosection of a P14 Sox2 Egfp/+ pituitary. Schematic of the workflow used for bulk RNA-sequencing analysis of Sox2 + and Sox2 − cells. Genome browser views of reads aligning to the Sox2 and Pit1 loci in the positive and negative fractions indicating good separation of the EGFP + population. Scale bar: 50 µm. ( B ) Sox2 + cells express a significant enrichment in markers associated with epithelial-to-mesenchymal transition (EMT), adherens, and tight junctions, consistent with their epithelial nature. Gene set enrichment analysis (GSEA) plots and immunofluorescence staining against E-Cadherin (adherens junction marker) and ZO1 (tight junction marker) in the marginal zone epithelium at P14. Scale bar: 50 µm. See for full GSEA gene lists. ( C ) Sox2 + cells express a significant enrichment in several signalling pathways, shown with respective GSEA plots. See for full GSEA gene lists. ( D ) Bar charts showing the FPKM values of components of the LGR/RNF43/ZNRF3/R-spondin module in the Sox2 + and Sox2 − fractions and the distribution of the Frizzled receptors. GSEA plot for components of the WNT pathway. Validation of sequencing: (i) mRNA in situ hybridisation with specific probes against Lgr4 (blue) and Sox2 (red) in P14 pituitaries showing co-expression. (ii) Double mRNA in situ hybridisation against Fzd4 (blue) and Sox2 (red) indicating co-expression in both the marginal zone epithelium and parenchymal Sox2 + cells. Boxed regions are magnified. Scale bars: 250 µm and 50 μm in boxed inserts. (iii) mRNA in situ hybridisation against Rspo1 , Rspo2 , Rspo3 , and Rspo4 in sagittal sections of wild-type pituitaries at P14. Boxed regions are magnified, only Rspo4 is detected. Scale bars: 250 µm and 100 μm in boxed inserts.

    Techniques Used: Expressing, RNA Sequencing, Immunofluorescence, Staining, Marker, Biomarker Discovery, Sequencing, In Situ, Hybridization


    Figure Legend Snippet:

    Techniques Used: Isolation, Sequencing, RNAscope, Positive Control, Negative Control, Recombinant, Staining, Software

    Related Articles

    Sequencing:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    RNAscope:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Expressing:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    RNA Sequencing:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Immunofluorescence:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Staining:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Marker:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Biomarker Discovery:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    In Situ:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Hybridization:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Isolation:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Positive Control:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Negative Control:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Recombinant:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Software:

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.59142 12 of 23 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Mouse Biotinylated (Goat Biotinylated) Abcam ab6788, RRID:AB_954885 IF(1:400) Sequencebased reagent RNAscope probe M. musculus Axin2 Advanced Cell Diagnostics 400331 Sequencebased reagent RNAscope probe M. musculus Lef1 Advanced Cell Diagnostics 441861 Sequencebased reagent RNAscope probe M. musculus Wls Advanced Cell Diagnostics 405011 Sequencebased reagent RNAscope probe M. musculus Rspo1 Advanced Cell Diagnostics 401991 Sequencebased reagent RNAscope probe M. musculus Rspo2 Advanced Cell Diagnostics 402001 Sequencebased reagent RNAscope probe M. musculus Rspo3 Advanced Cell Diagnostics 402011 Sequencebased reagent RNAscope probe M. musculus Rspo4 Advanced Cell Diagnostics 402021 Sequencebased reagent RNAscope probe M. musculus Lgr4 Advanced Cell Diagnostics 318321 Sequencebased reagent RNAscope probe M. musculus Wnt9a Advanced Cell Diagnostics 405081 Sequencebased reagent RNAscope probe M. musculus Wnt2 Advanced Cell Diagnostics 313601 Sequencebased reagent RNAscope probe M. musculus Wnt5a Advanced Cell Diagnostics 316791 Sequencebased reagent RNAscope probe eGFP Advanced Cell Diagnostics 400281 Sequencebased reagent RNAscope probe M. musculus Jun Advanced Cell Diagnostics 453561 Sequencebased reagent RNAscope probe M. musculus Axin2 (Channel 2) Advanced Cell Diagnostics 400331-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 (Channel 2) Advanced Cell Diagnostics 401041-C2 Sequencebased reagent RNAscope probe eGFP (Channel 2) Advanced Cell Diagnostics 400281-C2 Sequencebased reagent RNAscope probe M. musculus Sox2 Advanced Cell Diagnostics 401041 Sequencebased reagent RNAscope probe M. musculus Pou1f1 Advanced Cell Diagnostics 486441 Sequencebased reagent RNAscope probe Duplex Positive Control Ppib-C1, Polr2a-C2 Advanced Cell Diagnostics 321641 Sequencebased reagent RNAscope probe Duplex Negative Control DapB (both channels) Advanced Cell Diagnostics 320751 Sequencebased reagent RNAscope probe Singleplex Positive Control Ppib Advanced Cell Diagnostics 313911 Continued on next page Russell et al. eLife 2021;10:e59142.

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells
    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .



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    rnascope probe m. musculus rspo1  (Advanced Cell Diagnostics Inc)
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    Advanced Cell Diagnostics Inc rnascope probe m. musculus rspo1
    ( A ) Native EGFP protein expression in frontal cryosection of a P14 Sox2 Egfp/+ pituitary. Schematic of the workflow used for bulk RNA-sequencing analysis of Sox2 + and Sox2 − cells. Genome browser views of reads aligning to the Sox2 and Pit1 loci in the positive and negative fractions indicating good separation of the EGFP + population. Scale bar: 50 µm. ( B ) Sox2 + cells express a significant enrichment in markers associated with epithelial-to-mesenchymal transition (EMT), adherens, and tight junctions, consistent with their epithelial nature. Gene set enrichment analysis (GSEA) plots and immunofluorescence staining against E-Cadherin (adherens junction marker) and ZO1 (tight junction marker) in the marginal zone epithelium at P14. Scale bar: 50 µm. See for full GSEA gene lists. ( C ) Sox2 + cells express a significant enrichment in several signalling pathways, shown with respective GSEA plots. See for full GSEA gene lists. ( D ) Bar charts showing the FPKM values of components of the LGR/RNF43/ZNRF3/R-spondin module in the Sox2 + and Sox2 − fractions and the distribution of the Frizzled receptors. GSEA plot for components of the WNT pathway. Validation of sequencing: (i) mRNA in situ hybridisation with specific probes against Lgr4 (blue) and Sox2 (red) in P14 pituitaries showing co-expression. (ii) Double mRNA in situ hybridisation against Fzd4 (blue) and Sox2 (red) indicating co-expression in both the marginal zone epithelium and parenchymal Sox2 + cells. Boxed regions are magnified. Scale bars: 250 µm and 50 μm in boxed inserts. (iii) mRNA in situ hybridisation against <t>Rspo1</t> , Rspo2 , Rspo3 , and Rspo4 in sagittal sections of wild-type pituitaries at P14. Boxed regions are magnified, only Rspo4 is detected. Scale bars: 250 µm and 100 μm in boxed inserts.
    Rnascope Probe M. Musculus Rspo1, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Native EGFP protein expression in frontal cryosection of a P14 Sox2 Egfp/+ pituitary. Schematic of the workflow used for bulk RNA-sequencing analysis of Sox2 + and Sox2 − cells. Genome browser views of reads aligning to the Sox2 and Pit1 loci in the positive and negative fractions indicating good separation of the EGFP + population. Scale bar: 50 µm. ( B ) Sox2 + cells express a significant enrichment in markers associated with epithelial-to-mesenchymal transition (EMT), adherens, and tight junctions, consistent with their epithelial nature. Gene set enrichment analysis (GSEA) plots and immunofluorescence staining against E-Cadherin (adherens junction marker) and ZO1 (tight junction marker) in the marginal zone epithelium at P14. Scale bar: 50 µm. See for full GSEA gene lists. ( C ) Sox2 + cells express a significant enrichment in several signalling pathways, shown with respective GSEA plots. See for full GSEA gene lists. ( D ) Bar charts showing the FPKM values of components of the LGR/RNF43/ZNRF3/R-spondin module in the Sox2 + and Sox2 − fractions and the distribution of the Frizzled receptors. GSEA plot for components of the WNT pathway. Validation of sequencing: (i) mRNA in situ hybridisation with specific probes against Lgr4 (blue) and Sox2 (red) in P14 pituitaries showing co-expression. (ii) Double mRNA in situ hybridisation against Fzd4 (blue) and Sox2 (red) indicating co-expression in both the marginal zone epithelium and parenchymal Sox2 + cells. Boxed regions are magnified. Scale bars: 250 µm and 50 μm in boxed inserts. (iii) mRNA in situ hybridisation against Rspo1 , Rspo2 , Rspo3 , and Rspo4 in sagittal sections of wild-type pituitaries at P14. Boxed regions are magnified, only Rspo4 is detected. Scale bars: 250 µm and 100 μm in boxed inserts.

    Journal: eLife

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

    doi: 10.7554/eLife.59142

    Figure Lengend Snippet: ( A ) Native EGFP protein expression in frontal cryosection of a P14 Sox2 Egfp/+ pituitary. Schematic of the workflow used for bulk RNA-sequencing analysis of Sox2 + and Sox2 − cells. Genome browser views of reads aligning to the Sox2 and Pit1 loci in the positive and negative fractions indicating good separation of the EGFP + population. Scale bar: 50 µm. ( B ) Sox2 + cells express a significant enrichment in markers associated with epithelial-to-mesenchymal transition (EMT), adherens, and tight junctions, consistent with their epithelial nature. Gene set enrichment analysis (GSEA) plots and immunofluorescence staining against E-Cadherin (adherens junction marker) and ZO1 (tight junction marker) in the marginal zone epithelium at P14. Scale bar: 50 µm. See for full GSEA gene lists. ( C ) Sox2 + cells express a significant enrichment in several signalling pathways, shown with respective GSEA plots. See for full GSEA gene lists. ( D ) Bar charts showing the FPKM values of components of the LGR/RNF43/ZNRF3/R-spondin module in the Sox2 + and Sox2 − fractions and the distribution of the Frizzled receptors. GSEA plot for components of the WNT pathway. Validation of sequencing: (i) mRNA in situ hybridisation with specific probes against Lgr4 (blue) and Sox2 (red) in P14 pituitaries showing co-expression. (ii) Double mRNA in situ hybridisation against Fzd4 (blue) and Sox2 (red) indicating co-expression in both the marginal zone epithelium and parenchymal Sox2 + cells. Boxed regions are magnified. Scale bars: 250 µm and 50 μm in boxed inserts. (iii) mRNA in situ hybridisation against Rspo1 , Rspo2 , Rspo3 , and Rspo4 in sagittal sections of wild-type pituitaries at P14. Boxed regions are magnified, only Rspo4 is detected. Scale bars: 250 µm and 100 μm in boxed inserts.

    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Techniques: Expressing, RNA Sequencing, Immunofluorescence, Staining, Marker, Biomarker Discovery, Sequencing, In Situ, Hybridization

    Journal: eLife

    Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

    doi: 10.7554/eLife.59142

    Figure Lengend Snippet:

    Article Snippet: Sequence-based reagent , RNAscope probe M. musculus Rspo1 , Advanced Cell Diagnostics , 401991 , .

    Techniques: Isolation, Sequencing, RNAscope, Positive Control, Negative Control, Recombinant, Staining, Software